Avidin plate assay system for enzymatic characterization of a histone lysine methyltransferase

Interrogation of SET7/9 Histone Lysine Methyltransferase Using a High Throughput Fluorescence Polarization Screening Assay

There continues to be increasing interest in epigenetic mechanisms of control that alter chromatin structure and influence gene regulation. Posttranslational modifications (PTMs) of histones have been shown to provide a complex mechanism of influencing chromatin structure that play a primary role in many DNA regulatory processes1. Structural studies of nucleosomes have shown that highly basic h...

Purification and Functional Characterization of SET8, a Nucleosomal Histone H4-Lysine 20-Specific Methyltransferase

BACKGROUND Covalent modifications of histone N-terminal tails play fundamental roles in regulating chromatin structure and function. Extensive studies have established that acetylation of specific lysine residues in the histone tails plays an important role in transcriptional regulation. Besides acetylation, recent studies have revealed that histone methylation also has significant effects on h...

Biological Functions of Dot1l, the Histone H3 Lysine 79 Methyltransferase

Characterization of the grappa gene, the Drosophila histone H3 lysine 79 methyltransferase.

We have identified a novel gene named grappa (gpp) that is the Drosophila ortholog of the Saccharomyces cerevisiae gene Dot1, a histone methyltransferase that modifies the lysine (K)79 residue of histone H3. gpp is an essential gene identified in a genetic screen for dominant suppressors of pairing-dependent silencing, a Polycomb-group (Pc-G)-mediated silencing mechanism necessary for the maint...

Histone lysine methyltransferase SETD8 promotes carcinogenesis by deregulating PCNA expression.

Although the physiologic significance of lysine methylation of histones is well known, whether lysine methylation plays a role in the regulation of nonhistone proteins has not yet been examined. The histone lysine methyltransferase SETD8 is overexpressed in various types of cancer and seems to play a crucial role in S-phase progression. Here, we show that SETD8 regulates the function of prolife...